Journal of Plant Biochemistry & Physiology

Journal of Plant Biochemistry & Physiology
Open Access

ISSN: 2329-9029

+44 1478 350008

Njagi JM

Njagi JM


  • Research Article
    Regeneration of Kenyan Cassava (Manihot Esculenta Crantz) Genotypes
    Author(s): Ngugi MP, Oduor RO, Omwoyo RO, Njagi JM, Mgutu AJ and Cheruiyot RCNgugi MP, Oduor RO, Omwoyo RO, Njagi JM, Mgutu AJ and Cheruiyot RC

    A reproducible regeneration system based on direct somatic embryogenesis is described for Kenya cassava lines. Cassava plants were regenerated at high frequency by inducing shoot primordial on explants derived from cotyledons of cassava somatic embryos.Various parameters were evaluated on their effects on callus induction, somatic embryogenesis, maturation and germination of somatic embryos as well as recovery of regenerated plantlets. Immature leaf lobes were used as explants for somatic embryogenesis. Three Kenyan cassava genotypes viz; Adhiambo Lera, Kibanda Meno and Serere along with a model cultivar, TMS 60444 were used this system. Remarkable regeneration frequencies were observed in all the evaluated genotypes with Adhiambo Lera showing the best responses. As a result, a highly efficient plant regeneration protocol via germinat.. View More»
    DOI: 10.4172/2329-9029.1000147

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