Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
ISSN: 2157-7013
Ning Zhang
University Cancer Hospital,
No.52 Fucheng Road, Haidian District Beijing, 100142
Indiana
Research Article
Application of Hypoxia to Establish Epithelial-Mesenchymal Transition Models in
Renal Cell Carcinoma Cell Lines
Author(s): Baoan Hong, Changhua Zhou, Xin Du, Siqi Chen, Xiaohu Deng, Shayiremu Duoerkun, Qing Li, Yong Yang, Kan Gong and Ning ZhangBaoan Hong, Changhua Zhou, Xin Du, Siqi Chen, Xiaohu Deng, Shayiremu Duoerkun, Qing Li, Yong Yang, Kan Gong and Ning Zhang
Purpose: To establish epithelial-mesenchymal transition (EMT) models in renal cell carcinoma cell lines by CoCl2-induced hypoxia.
Materials and methods: The renal cell carcinoma cell lines A498 and 786-O were used in the experiment and CoCl2 was used to simulate hypoxia. The cancer cells were cultured with different concentrations of CoCl2. Morphology and cyto-activity changes were detected to obtain the optimal concentration of CoCl2 for simulating hypoxia. After CoCl2 treatment, the cells were subjected to Western blot analysis to test the expression of HIF-1α and the changes of EMT-related molecules (E-cadherin, fibronectin).
Results: Cell conjunctions of CoCl2-treated groups were loose and scattered compared to the control. The effect of CoCl2 on A498 cell viability was not distinct at a low dosage, but when .. View More»
DOI:
10.4172/2157-7013.1000242