Journal of Hematology & Thromboembolic Diseases
Open Access
ISSN: 2329-8790
+44 7868 792050
ISSN: 2329-8790
+44 7868 792050
Nicolas Novitzky
South Africa
Research Article
Cancer/Testis Antigen Expression Panel Incorporating MAGEC1 and BAGE2 Predicts Multiple Myeloma Disease Stage and Severity
Author(s): Karen Shires, Andrea Teuchert, Kirsty Wienand, Iva Shankland and Nicolas NovitzkyKaren Shires, Andrea Teuchert, Kirsty Wienand, Iva Shankland and Nicolas Novitzky
To provide a single molecular assay that could be used to easily stage Multiple Myeloma patients at diagnosis, we investigated the association between the simultaneous expression of 7 Multiple Myeloma-associated Cancer/Testis Antigens and biochemical parameters that are currently used for disease staging. We analysed the mRNA expression of MAGEC1, MAGEA3/A6, BAGE2, PRAME, NYESO1, SSX2 and PAGE by qualitative reverse transcription PCR using RNA extracted from diagnostic bone marrow samples from 39 patients covering the Multiple Myeloma disease continuum and compared this to levels of key biochemical parameters at diagnosis. We found that the Cancer/Testis Antigen panel was expressed in a specific order that was specifically associated with the severity of disease. This allowed the Cancer/Testis Antigens expression profile to successfully place the patient clearly into either stage I or.. View More»
DOI:
10.4172/2329-8790.1000240