Cell & Developmental Biology
Open Access
ISSN: 2168-9296
+32-466-90-21-46
ISSN: 2168-9296
+32-466-90-21-46
Naznin Akhtar
Bangladesh
Research Article
Microbiological Quality Assessment at Different Stages of Frozen Amniotic Allograft Processing for Safe Tissue Banking Activities
Author(s): Md Arifuzzaman, Naznin Akhtar and SM AsaduzzamanMd Arifuzzaman, Naznin Akhtar and SM Asaduzzaman
Human Amniotic Membrane (HAM) is widely used as biological dressing material in reconstructive skin surgery, abdominal and vaginal reconstruction, plastic and cosmetic surgery and in ophthalmologic surgery. The objective of this study was to assess microbial quality of HAM during different stages of frozen HAM allograft processing and determination of antimicrobial susceptibility of isolated microorganisms. For this purpose, twelve amniotic sacs were collected from normal vaginal delivery of seronegative mother from Azimpur maternity, Dhaka, Bangladesh. Initial bioburden was determined by using Nutrient Agar (NA), McConkey Agar, EMB Agar, Potato Dextrose Agar (PDA). Total Viable Bacterial Count (TVBC) was calculated and Initial bacterial load was ranged from 39 to 5.25×103. No fungus was found. A total 28 bacterial isolates were selected. These bacter.. Read More»
DOI:
10.4172/2168-9296.1000197