Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
ISSN: 2161-0495
Naceur A. Boughattas
Tunisia
Research Article
Dosing-Time Dependence of Lethal Toxicity Induced by Nitroprusside in
Young Mice
Author(s): Mamane Sani, Hichem Sebai, Naceur A. Boughattas and Mossadok Ben-AttiaMamane Sani, Hichem Sebai, Naceur A. Boughattas and Mossadok Ben-Attia
Sodium nitroprusside (SNP) is a potent vasodilator and is commonly used as an antihypertensive agent in postoperative cardiac surgical patients. In this study, we investigated whether the SNP-induced lethality is influenced by the dosing-time. Mortality was induced by administering SNP in different doses (4.9, 5.4, 6.0, 6.6 mg/kg, i.p.) to Swiss albino mice, 2 to 8 weeks old, synchronized for at least 2 weeks by 12 h light (rest)/12 h dark (activity) span. Each dose was administered to comparable groups of animals (n= 6) at six different circadian times: 1, 5, 9, 13, 17, and 21 hours after light onset (HALO). Both ?2 and cosinor methods were used to analyze the time series data. Statistically significant dosing time-dependent changes were validated in the daily scale with the males more sensitive than females. A ultradian (? = 12 h) rhythm was detected by cosinor (P < 0.002) for ea.. View More»
DOI:
10.4172/2161-0495.1000114