Journal of Plant Biochemistry & Physiology
Open Access
ISSN: 2329-9029
+44 1478 350008
ISSN: 2329-9029
+44 1478 350008
Mohammed Jawhar
AECS, Damascus
Palestine
Research Article
An Efficient Throughput Protocol for Validation of Pathotyping by Enzymatic Mismatch Cleavage
Author(s): Mohammed Jawhar, Bradley Till, Amer Albaterni, Alaa Skiheita, Mohammed Imad Eddin Arabi and Nizar MirAliMohammed Jawhar, Bradley Till, Amer Albaterni, Alaa Skiheita, Mohammed Imad Eddin Arabi and Nizar MirAli
Early diagnosis of fungal pathotypes is one of the most important aspects for plant breeders. It provides a rapid means for selecting the correct mutant lines to ensure durability of disease resistance and also for proper field management practices. Traditional diagnostic tools for pathogens have been based on targeted cultures, PCRbased approaches, and/or phenotypic evaluation of disease response in specific plant genotypes. These methods detect only known pathogenic agents, can introduce bias, and can fail to recognize novel variants or races due to their narrow scope. While enzymatic mismatch cleavage has been described for many plant and animal species, the validate that technique for haploid microorganisms is also needed. In this work, optimized low cost method for pathotyping Fusarium oxysporum f. sp. cubense (Foc), the causal agent of fusarium wilt of banana (Musa spp.), was es.. View More»
DOI:
10.4172/2329-9029.1000203