Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
ISSN: 2161-0495
Maria Alice Cruz-Höfling
State University of Campinas (UNICAMP),
P.O. Box 6109, Zip Code 13083-865, Campinas, SP
Brazil
Research Article
Biochemical Characterization of a PLA2 Btae TX-I Isolated from Bothriopsis taeniata Snake Venom: A Pharmacological and Morphological Study
Author(s): Frey Francisco Romero-Vargas, Thalita Rocha, Maria Alice Cruz-Höfling, Lea Rodrigues-Simioni, Luis Alberto Ponce-Soto and Sergio MarangoniFrey Francisco Romero-Vargas, Thalita Rocha, Maria Alice Cruz-Höfling, Lea Rodrigues-Simioni, Luis Alberto Ponce-Soto and Sergio Marangoni
In this research a preliminary identification and biochemical and biological characterization of a PLA2 (Btae TX-I) from the venom of a viperid snake, Bothriopsis taeniata (Speckled forest pit viper) were obtained. Btae TX-I was purified by two chromatographic steps, molecular exclusion chromatography followed by analytical chromatography reverse phase HPLC. Molecular mass behaved as a homogeneous single chain protein on SDS–PAGE, confirmed by MALDI-TOF spectrometry, indicating a molecular mass of 13889.98 Da. Tryptic peptides were determined in tandem mass spectrometry and showed similarity with other myotoxic PLA2s. Btae TX-I belongs to the Asp49 PLA2 class, is enzymatically active in presence of a synthetic substrate and shows a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0 and 35–45°C. PLA2 activity in presence of Mn2+, Mg2+, Cd2+ and Zn2+ was.. View More»
DOI:
10.4172/2161-0495.1000197