Medical Safety & Global Health
Open Access
ISSN: 2574-0407
+44-20-4587-4809
ISSN: 2574-0407
+44-20-4587-4809
Majó N
Spain
Research Article
Assay of Several Inactivation Steps on West Nile Virus and H7N1 Highly Pathogenic Avian Influenza Virus Suspensions
Author(s): Xavier Abad, Majó N, Rosell R and Busquets NXavier Abad, Majó N, Rosell R and Busquets N
Objectives: In any research laboratory, precautionary measures must be taken in order to reduce or eliminate the potential risk of accidental infection by biosafety level 3 (BSL3) pathogens, as Highly Pathogenic Avian Influenza Virus (HPAI) or West Nile Virus (WNV). Appropriate virus inactivation procedures have to be set up to allow necessary further processing of specimens outside BSL3 facilities.
Methods: To study the elimination of WN and HPAI virus infectivity, the effect of different chemical and physical inactivation procedures on viral suspensions were investigated. A proper cell culture assay for each virus was performed to verify several treatments, which are commonly performed preceding transfer of materials outside of biocontainment, still allowing further investigations like sequencing or genome amplification.
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DOI:
10.4172/2167-0331.1000103