ISSN: 2574-0407
+44-77-2385-9429
LI Na
China
Research Article
Viral Penetration of High Efficiency Particulate Air Filter of Biological Safety Cabinet in microbiological Laboratory
Author(s): YU Long, DUAN Wei, WANG XiaoDong, WEN ZhanBo, LI Na and LI JinSongYU Long, DUAN Wei, WANG XiaoDong, WEN ZhanBo, LI Na and LI JinSong
Objective: Biological Safety Cabinets are the primary containment device used to protect the worker, environment and product from exposure to infectious agents within the laboratory. Spores of Bacillus subtilis are used as tracer agents to test Class II biological safety cabinets in NSF/ANSI Standard 49 and others. However, none of these standards characterizes the viral challenge tests.
Methods: One model bacterium and two different phages were selected to challenge two new Class II biological safety cabinets strictly conforming to the NSF/ANSI Standard 49 requirements.
Results: The two tested biological safety cabinets met the requirements of personnel, product, and crosscontamination protection test no matter which agent was used to challenge the system. However, the high efficiency particulate air filter leak testi.. View More»
DOI:
10.4172/2167-0331.1000102