Medical Safety & Global Health
Open Access
ISSN: 2574-0407
+44-20-4587-4809
ISSN: 2574-0407
+44-20-4587-4809
Laura JR
Tanzania
Research Article
Storage Effects on Sample Integrity of Environmental Surface Sampling Specimens with Bacillus anthracis Spores
Author(s): Allison KP, Heather AO, Laura JR, Judith NW and Matthew JAAllison KP, Heather AO, Laura JR, Judith NW and Matthew JA
The effect of packaging, shipping temperatures and storage times on recovery of Bacillus anthracis str. Sterne spores from swabs was investigated. Macrofoam swabs were pre-moistened, inoculated with Bacillus anthracis spores, packaged in primary containment or secondary containment before storage at -15°C, 5°C, 21°C, or 35°C for 0-7 days. Swabs were processed according to validated Centers for Disease Control/Laboratory Response Network culture protocols, and the percent recovery relative to a reference sample (T0) was determined for each variable. No differences were observed in recovery between swabs held at -15° and 5°C, (p ≥ 0.23). These two temperatures provided significantly better recovery than swabs held at 21°C or 35°C (all 7 days pooled, p ≤ 0.04). The percent recovery at 5°C was not signif.. View More»
DOI:
10.4172/2167-0331.S1-002