Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
ISSN: 2157-7013
Kenta Hatatani
Japan
Research Article
TCR Signaling via ZAP-70 Induced by CD3 Stimulation is More Active
Under Acidic Conditions
Author(s): Xin Wang, Kenta Hatatani, Yirong Sun, Toshihiko Fukamachi, Hiromi Saito and Hiroshi KobayashiXin Wang, Kenta Hatatani, Yirong Sun, Toshihiko Fukamachi, Hiromi Saito and Hiroshi Kobayashi
Although the pH values of blood and tissues are usually maintained in a narrow range around 7.4, some diseased areas, such as cancer nests, inflammatory loci, and infarction areas, are acidified. In the present study, the effect of extracellular acidic pH on TCR signaling was examined with human acute leukemia T cell line Jurkat cells because T cell infiltration is often observed in acidic diseased areas. The phosphorylation levels of CD3-ξ ZAP-70, and PLC-γ1 induced by OKT-3, anti-CD3 antibody, were higher at pH 6.3 than those at pH 7.6. The activation of PLC-γ1 induced by OKT-3 was further increased by the co-stimulation with CD28.6, anti- CD28 antibody, at pH 7.6, but not at pH 6.3. The level of cytosolic free calcium ions was increased to a higher level by the addition of OKT-3 at pH 6.3, compared with that by the addition of OKT-3 plus.. View More»
DOI:
10.4172/2157-7013.S15-002