Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
ISSN: 2161-0495
Jingchun Lu
Indiana
Research Article
Induction of Acetylcholinesterase Activity and Apoptosis in Carboxylesterase and Butyrylcholinesterase Knockout Mice Treated with Cocaine
Author(s): Jingchun Lu, Aziguli Yimaer, Ellen G. Duysen, Wenjun Sun and Wei JiangJingchun Lu, Aziguli Yimaer, Ellen G. Duysen, Wenjun Sun and Wei Jiang
Background: Cocaine is a commonly used illegal recreational drug and its consumption can produce various adverse health effects in animal and clinical studies. To date no information is available on whether exposed to cocaine will result in abnormally high plasma AChE activity in animals and whether it is characteristic of apoptosis. Our goals were to examine the relationship between enhanced AChE activity and cocaine-induced apoptosis and the possible underlying mechanisms.
Methods: For this purpose, carboxylesterase and butyrylcholinesterase deficient ES1-/-BChE-/- mice in strain C57BL/6 were treated intraperitoneally with 25 mg/kg cocaine daily for 8 days and sacrificed on day 9. Plasma AChE activity and body temperature were measured before and after treatment. Tissue sections from brain, heart, kidney, and liver were stained for AChE activ.. View More»
DOI:
10.4172/2161-0495.1000401