ISSN: 2469-9861
44-7452-259145
Janet Donaldson
Tanzania
Research Article
A Simple Dilute-and-Shoot Flow-Injection Mass Spectrometric Method for Quantification of Glycocholic Acid in Human Bile
Author(s): Raghavi Kakarla, Ramakrishna Reddy Voggu, Janet Donaldson and Baochuan GuoRaghavi Kakarla, Ramakrishna Reddy Voggu, Janet Donaldson and Baochuan Guo
Levels of glycocholic acid are elevated in the bile of cholangiocarcinoma patients, making it a potential biomarker for the disease. The utility of liquid chromatography mass spectrometry based methods for the quantification of glycocholic acid often suffers from time-consuming extraction steps, carryover, and unavailability of blank bile matrix. To overcome the problems, we developed a dilute-and-shoot flow-injection tandem mass spectrometry (standard addition-based) method for quantification of glycocholic acid in bile. Bile was first diluted, followed by spiking the glycocholic acid standard and internal standard into the diluted bile, which was then directly flow-injected (without chromatographic separation) into the electrospray ionization source. Detection was carried out in negative multiple reaction monitoring mode with m/z transitions set at 464.1?74.0 and 401.2?249.1 for glycoc.. Read More»
DOI:
10.4172/2469-9861.1000129