Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
ISSN: 2161-0495
Gorska M
Gdansk 80-211
Poland
Research Article
Effect of 2-Methoxyestradiol on Dephosphorylation of Neuronal Nitric Oxide Synthase in Osteosarcoma 143B Cells. An in vitro study
Author(s): Gorska M, Kuban-Jankowska A, Antoniewicz J and Wozniak MGorska M, Kuban-Jankowska A, Antoniewicz J and Wozniak M
Objective: 2-methoxyestradiol, an endogenous metabolite of anticancer agent, has been evaluated in ongoing advanced phases of clinical trials as an anticancer agent. The aim of the study was to determine the impact of 2- methoxyestradiol on phosphorylation status of neuronal nitric oxide synthase at position of serine 847. We determined also influence of the compound on the gene and protein expressions, phosphorylation status of PP2A phosphatase.
Methods: In the current study we used western blotting, real time PCR, and ELISA assays.
Results: We demonstrated that 2-methoxyestradiol decreases the level of neuronal nitric oxide synthase phosphorylated at position of serine 847 referred to as inactive pool of enzyme. The expression of PP2CA gene was down-regulated by 2-methoxyestradiol at concentration of 1 μM. 10 .. View More»
DOI:
10.4172/2161-0495.1000240