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Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against Tumor Cell Lines without Systemic Alterations in BALB/c Mice.
Author(s): Hapon MB, Hapon MV, Persia FA, Pochettino A, Lucero GS and Gamarra-Luques CHapon MB, Hapon MV, Persia FA, Pochettino A, Lucero GS and Gamarra-Luques C
Prosopis strombulifera (Lam.) Benth. is a rhizomatous shrub native to the northern and central zones of Argentina. The analgesic and antibiotic properties of this plant had been demonstrated but there are no previous reports of its cytotoxic activity. The goal of this work was to analyze the cytotoxic activity of P. strombulifera against HCT-116 and MCF-7 cell lines, and to evaluate toxic systemic effects in BALB/c mice. Changes induced by the aqueous extract from leaves on tumoral cell lines were studied by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) tetrazolium reduction assay, Trypan blue dye exclusion assay, optical microscopy, Western Blot analysis of PCNA and cPARP, LDH activity, Ames´ test and clonogenic survival. Oral sub-chronic toxicity was assessed on BALB/c mice at concentrations up to 150 mg/animal/day. Analyses included animal/organs weight;.. Read More»