Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495

+44 7868 792050

Dharmendra Kumar Maurya

Dharmendra Kumar Maurya
Bhabha Atomic Research Centre,

  • Research Article
    ColonyCountJ: A User-Friendly Image J Add-on Program for Quantification of Different Colony Parameters in Clonogenic Assay
    Author(s): Dharmendra Kumar MauryaDharmendra Kumar Maurya

    Objective: There are several commercial automatic colony counters available but they are relatively expensive and associated with several quantifications related problems. Some of the key problems in automated colony counting are clustering of colonies and edge effects. Thus the main objective of this study is to develop a user friendly program for determining colony parameters of clonogenic assay. Methods: In the present study, a watershed algorithm was employed along with other ImageJ tools to develop ImageJ based add-on program i.e ColonyCountJ to calculate various colony parameters. To test this program, we have used colonies obtained after exposure of MCF-7 cells to different doses of γ-radiation. Results: The results obtained using this program were compared with the manual counting as well as with automated counting provided by Oxford Optronix GelCount.. View More»
    DOI: 10.4172/2161-0495.1000358

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