Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Dhanya Saraswathiamma
United Arab Emirates
Research Article
Effects of Selected Inhibitors of Protein Kinases and Phosphatases on Cellular Respiration: An In Vitro Study
Author(s): Saeeda Almarzooqi, Alia Albawardi, Ali S. Alfazari, Dhanya Saraswathiamma, Hidaya Mohammed Abdul-Kader, Sami Shaban, Robert Mallon and Abdul-Kader SouidSaeeda Almarzooqi, Alia Albawardi, Ali S. Alfazari, Dhanya Saraswathiamma, Hidaya Mohammed Abdul-Kader, Sami Shaban, Robert Mallon and Abdul-Kader Souid
Inhibitors of protein kinases/phosphatases are known to alter cellular metabolism. Effects of these rapidly identified small molecules on cellular respiration (mitochondrial O2 consumption) have not been adequately investigated, especially in healthy organs. This in vitro study measured cellular respiration in tissues from C57BL/6 mice with and without GSK2126458 (PI3K/mTOR inhibitor), BEZ235 (PI3K/mTOR inhibitor), GDC0980 (PI3K/mTOR inhibitor), GSK1120212 (trametinib, MEK inhibitor), sorafenib, regorafenib (multikinase inhibitors), and cyclosporine (calcineurin inhibitor). Cellular respiration was measured by the phosphorescence oxygen analyzer, aided by the O2 probe Pd(II)-meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin. Cyanide inhibited O2 consumption, confirming the oxidation occurred in the respiratory chain. Renal cellular respiration decreased 26-34% in the presence of 10 &.. View More»
DOI:
10.4172/2161-0495.1000212