Journal of Plant Biochemistry & Physiology

Journal of Plant Biochemistry & Physiology
Open Access

ISSN: 2329-9029

+3225889658

Dagnachew Bekele

Dagnachew Bekele
Ethiopian Institute of Agricultural Research,
Addis Ababa
Ethiopia

Publications
  • Review Article
    Applications of Virus Induced Gene Silencing (VIGS) in Plant Functional Genomics Studies
    Author(s): Dagnachew Bekele, Kassahun Tesfaye and Asnake FikreDagnachew Bekele, Kassahun Tesfaye and Asnake Fikre

    Virus induced gene silencing (VIGS) is an effective technology that exploits an antiviral defense mechanism in plants. It is a recently developed gene transcript suppression technique for characterizing the function of plant genes. VIGS is rapid, efficient and specific system for transient gene silencing. The major steps in VIGS includes; engineering viral genomes to the appropriate viral vector to incorporate fragments of host genes that are targeted to be silenced, infecting the appropriate plant hosts and silencing the target genes as part of the defense mechanism of the plant against virus infection. The VIGS vector is a recombinant virus engineered to be able to carry a piece an endogenous gene from the host. During infection with the modified vector, the host’s defense reaction will be induced against the cloned host gene; a loss of function phenotype makes it possible to .. Read More»
    DOI: 10.4172/2329-9029.1000229

    Abstract PDF

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