Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495

+44 20 3868 9735

Byung Sun Yoo

Byung Sun Yoo

Suwon, Kyonggi-Do

  • Research Article
    Benzopyrene Inhibits Differentiation of Human SH-SY5Y Neuroblastomain Culture in Cells
    Author(s): Do Eun Kim and Byung Sun YooDo Eun Kim and Byung Sun Yoo

    Benzopyrene (BaP) is polycyclic aromatic hydrocarbon (PAH), and is chemically modified in the animal body to form a number of metabolites that may elicit a various toxicity. However, the effect of BaP on neuroblastoma differentiation has remained unclear. We have studied the effect of BaP on neurite outgrowth using human SH-SY5Y neuroblastoma cells induced to differentiate by all-trans-retinoic acid (RA). Whereas BaP, at a concentration of 1 μM, had no significant effect on the viability of differentiating SH-SY5Y cells, the neurite outgrowth of differentiating SHSY5Y cells 48 h after BaP treatment was significantly inhibited. Treatment of RA-stimulated differentiating SH-SY5Y cells with 0.1–3 μM BaP resulted in decreased level of cross-reactivities with tissue glutaminase (TGase) antibody in a dose-dependent manner. To investigate the involvement of AhR signaling in the i.. Read More»
    DOI: 10.4172/2161-0495.1000362

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