Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Byeong-Gon Kim
Republic of Korea
Research Article
Claudin 5 Transcripts Following Acrolein Exposure Affected by Epigenetic
Enzyme
Author(s): Kuk-Young Moon, Pureun-Haneul Lee, Byeong-Gon Kim, Moo-Kyun Park and An-Soo JangKuk-Young Moon, Pureun-Haneul Lee, Byeong-Gon Kim, Moo-Kyun Park and An-Soo Jang
Background and objective: Acrolein is a highly reactive α, β-unsaturated aldehyde and a respiratory irritant that is ubiquitously present in the environment but that can also be generated endogenously at sites of inflammation. Apical junctional protein claudin 5 can be affected by acrolein. This study aimed to determine the impact of aberrant expression of DNMT1, DNMT3b, MBD3, and MeCP2 on acrolein induced claudin 5.
Methods: EA.hy926 cell lines were exposed to acrolein 30 nm for 1 h, 2 h, and 4 h. Epigenetic enzyme such as DNMT1, DNMT3b, MBD3 and MeCP2 were quantified in the cell line using real time PCR. Claudin 5 methylation was checked by methtyl specific PCR.
Results: After acrolein 30 nm exposure, MBD3 and MeCP2 transcript were decreased at 1 h and increased at 2 h and 4 h compared to control. DNMT3b transcript was decreased at 1 h, 2 h, and 4 h followin.. View More»
DOI:
10.4172/2161-0495.1000268