Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Antoinette Defaux
Switzerland
Research Article
Delta-9-Tetrahydrocannabinol (THC) Protects Partly against
Demyelination by Modulating the Inflammatory Response: An In Vitro
Study in Aggregating Brain Cell Cultures
Author(s): Antoinette Defaux, Mariano Schiffrin, Lorianne Vorlet-Fawer, Amelie Spiehlmann, Christian Giroud and Florianne Monnet-TschudiAntoinette Defaux, Mariano Schiffrin, Lorianne Vorlet-Fawer, Amelie Spiehlmann, Christian Giroud and Florianne Monnet-Tschudi
Delta 9-tetrahydrocannabinol (THC) has been proposed as therapeutic agent in the treatment of multiple sclerosis. In the present study, we examined whether a modulation of brain inflammation by THC may protect against demyelination. Myelinating aggregating brain cell cultures were subjected to demyelination by a repeated treatment (3x) with the two inflammatory agents interferon-γ (IFN-γ) and lipopolysaccharide (LPS). The effects of THC on an acute inflammatory response were also examined by treating the aggregates with a single application of the two inflammatory agents. THC effects on the demyelinating process and on several mediators of the inflammatory response were analyzed. THC treatment partially prevented the decreased immunoreactivity for MBP, and the decrease in MBP content measured by immunoblotting. It prevented IFN-γ + LPS-induced microglial reactivity; .. View More»
DOI:
10.4172/2161-0495.S6-002