Advances in dairy Research

Advances in dairy Research
Open Access

ISSN: 2329-888X


Comparative Efficacy Evaluation of Six Brands of Amoxicillin against S. aureus Isolated from Subclinical Mastitic Milking Dairy Cows in Bishoftu

Solomon Berhe, Takele Beyene, Tariku Jibat and Woldemariyam Fanos Tadesse

Bovine mastitis is inflammation of the mammary glands that interferes with the normal flow and quality of milk. S. aureus is the most important pathogen among Staphylococci species related to subclinical mastitis (SCM) in dairy cows. Antibiotics must be safe, effective and of acceptable quality to be used in both human and veterinary medicines. The study was aimed to isolate S. aureus from California mastitis test (CMT) positive dairy cows and to evaluate the efficacy of the six brands of amoxicillin against S. aureus. Purposive sampling with a cross-sectional study design was conducted from February 2016 to April 2016 in five dairy farms found in Bishoftu town. A total of 162 dairy cows were examined using California mastits test (CMT) and out of this 112 (69%) were found positive and from this 30 (26.78%) isolates of S. aureus were recorded. The highest and the lowest prevalence of the California mastitis test(CMT) positive was found in Prime (100%) and Tseday (36.84%) farm respectively. The highest and the lowest prevalence of the S. aureus isolate were found in Prime (50%) and college of veterinary medicine and agriculture (10%) farm respectively. In vitro drug efficacy against the bacterial isolates was determined by comparing the zone of inhibition obtained from clinical and laboratory standards institute (CLSI) by using disc diffusion method. The comparative efficacy between the brands was evaluated by measuring zone of inhibition and was interpreted as resistant, intermediate and susceptible. S. aureus isolate were 100% resistant to the six different brands of amoxicillin. Generally it is concluded that S. aureus is among the major causative agent of subclinical mastitis in five dairy farms of the study area.The isolates were also resistance to amoxicillin brands indicating the need of other alternative and effective antibiotics.