Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Jana Pisarcikova
Institute of Animal Physiology-Slovak Academy of Sciences, Slovakia
Auburn University Harrison School of Pharmacy, USA
Posters & Accepted Abstracts: J Chromatogr Sep Tech
Due to the fact that the use of antibiotics as growth promoters in the European Union has been prohibited since 2006, the application of essential oils in food preservation, animal production and in agriculture is growing in last two decades. The main expected consequence of the ban is to minimize the amount of antibiotics used in animal production owing to concerns about consumer safety. In our experiment, the thymol metabolites ��? thymol sulfate was quantified and thymol glucuronide was identified in plasma, liver and duodenal wall of broiler chickens after feeding with a Thymus vulgaris L. essential oil at different concentrations (0.01%, 0.05%, 0.1% w/w) using UHPLC coupled with accurate-mass QTOF-MS. The aim of this work was to develop and validate a method in a direct way without previous enzymatic hydrolysis of thymol metabolites. The validation was performed according to the ICH guidelines and the parameters for validation were fully determined for each biological matrix; plasma, liver and duodenal wall as follows: selectivity, linearity, accuracy and precision, matrix effect, recovery, stability, limit of detection and limit of quantification. UHPLC-QTOF-MS was shown to be highly sensitive and selective for metabolite profiling and thymol metabolites were confirmed by MS software according to molecular formula, score, mass error and double bond equivalent.