Journal of Chromatography & Separation Techniques
Open Access

The possibilities of amino acid ionic liquids as a chiral selectors at separation of enantiomers of amino acids and β-blockers

World Congress on Chromatography

September 21-23, 2016 Amsterdam, Netherlands

E A Kolobova, L A Kartsova, E A Bessonova, E V Alopina and E Safonova

Saint-Petersburg State University, Russia

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Chiral separation is an important subject in science as well as in technology. Various chiral selectors, such as cyclodextrins, antibiotics and crown ethers have been widely used in separation of enantiomers because of their chiral recognition abilities. However, the application of many current chiral selectors is often limited due to their low solubility, difficult synthesis, thermal instability as well as high cost. In addition, most of selectors need to be dissolved in other solvents or in a solvent system as work solution. Therefore, using chiral ionic liquids as chiral selector is promising. Amino acids ionic liquids [C4Mim] [L-Pro], [C8Mim][L-Pro], [C12Mim][L-Pro] were synthesized and characterized by NMR-spectrums. In the course of the optimization of chiral separation conditions were varied: the composition background electrolyte, the structure of selectors and the concentration of ionic liquid in the modified buffer electrolytes. IL [C4Mim][L-Pro] was the most effective ionic liquid for the chiral amino acids separation under ligand-exchange capillary electrophoresis. The highest enantioselectivity factors (�?±=5.2) were achieved for tryptophane when complexing metal was cupper. Synergetic effect was observed at simultaneously addition 2-OH-proplyl-�?²-cyclodextrin and chiral IL to the running buffer as a result enantiomers of propranolol and carvedilol were separated. Analysis of drug formulation â�?�?Carvedilol zentivaâ�? and â�?�?Anaprilinâ�? was performed.

Biography :

Email: ekatderyabina@mail.ru