Temperature controlled liquid phase microextraction, in-situ deri | 54209
Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

+44 1300 500008

Temperature controlled liquid phase microextraction, in-situ derivatization for determination of estrogens in water by gas chromatography-tandem mass spectrometry

World Congress on Chromatography

September 21-23, 2016 Amsterdam, Netherlands

Maw-Rong Lee, Chung-Yu Chen and Yi-Yu Chen

National Chung Hsing University, Taiwan

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Estrogens, one group of endocrine disrupting chemicals (EDCs), can easily accumulate in the human body and interfere with organismâ�?�?s endocrine system at low concentration. The trace estrogens in environmental water system have attracted scientistsâ�?�? and human attention. Therefore, to develop a simple, rapid, and high sensitive analytical method for determination of the trace estrogens in environmental water is important for environmental safety. In this study, a novel temperature-controlled liquid phase microextraction (TC-LPME) by in-situ derivatization coupled to gas chromatography-tandem mass spectrometry (GC-MS/MS) for analyzing trace estrogens including estrone, 17�?±-estradiol, 17�?±-ethinylestradiol, mestranol, diethylstilbestrol, and steinheim in water matrix has been developed. An aliquot of 11.25 mL water sample with pH 7 and containing 5% sodium chloride reacted with 3.75 mL dansyl chloride (10 �?¼g/mL) as derivatization agent has been prepared. After derivatization for 15 minutes at 60�?ºC, the derivatives were extracted by liquid phase microextraction with octanol as extraction solvent and the extractant was analyzed by GC-MS/MS. The linearity of the proposed method was 0.05 to 50 ng/mL; with a coefficient of determination above 0.9940. The limits of detection (LODs) of target estrogens were between 0.3 and 1.1 ng/mL. The intra-day and inter-day precisions were between 1.2 and 10.8% and 2.9 and 17.6%, respectively. The recovery of the proposed method ranged 84.9 and 107.8%. The results demonstrated that the proposed TC-LPME-GC-MS/MS method is rapid, simple, high sensitive and high selective method for the determination of trace estrogens in environmental water matrix.

Biography :

Maw-Rong Lee has completed his MS from University of Florida. He is Dean of College of Science and Distinguished Professor of Department of Chemistry at the National Chung Hsing University, Taiwan. He has published more than 80 papers in reputed journals.