Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Mellissa Graewert and Dmitri Svergun
EMBL Hamburg Outstation, Germany
Scientific Tracks Abstracts: J Chromatogr Sep Tech
Small angle X-ray scattering (SAXS) is a universal and powerful method to analyze proteins and other macromolecules in solution, in a broad range of sizes and conditions. The synergistic improvement in hardware as well as software over the last decade has transformed SAXS into a high-through put technique, which became highly attractive for the pharmaceutical industry. SAXS provides direct insights in the quaternary state; however, it is often hampered by inherent sample polydispersity. At EMBL��?s P12 beamline (@Petra III, DESY, Hamburg, Germany), we are developing novel in-line purification systems such as the implementation of an extended size exclusion chromatography set-up for the parallel biophysical and SAXS characterization on separated components. The eluting protein is sufficiently concentrated and pure, so that SAXS data can be directly collected and used for structural and biophysical studies. Modes of access to this set-up (including European funded translational activities such as iNEXT and industrial service provision) are discussed.
Melissa Graewert is a Structural Biologist. Currently, she works at EMBL's Outstation in Hamburg, Germany located at PETRA III, one of the most brilliant storagering- based X-ray radiation sources in the world. Her expertise includes “Biophysical and structural characterization of proteins”. Her main research focus is on “The implementation and constant development of small angle X-ray scattering as an emerging technique for the characterization of biological therapeutics such as monoclonal antibodies”.