Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Md Ahsan Habib
Posters-Accepted Abstracts: J Chromatogr Sep Tech
A scientific field that deals metabolites is termed as metabolomics. Specifically, metabolomics is the �??systematic study of the unique chemical fingerprints that specific cellular processes leave behind�?�. For long time, jute (Corchorus olitorius Linn.) leaves have been used as an edible vegetable in some regions (such as Asia, Middle East, and Africa) of the world because the leaves contain therapeutically important metabolites which are considered as beneficial for human health. Such metabolites are beta-carotene, alpha-tocopherol, oleanolic acid, coumarinetc where alpha-tocopherol acts as an antioxidant. In this study, attempts have been made to investigate the metabolites present in jute leaves by mass spectrometry through fabrication of hybrid ion source of ESI and DBD glow discharge. A hybrid ion source could be useful to detect both the polar and non-polar metabolites present in biological samples simultaneously. In this work, jute leaves were taken as model samples and analyzed using a hybrid ion source of ESI and glow discharge. About 8 weeks aged jute leaves were collected from a jute field of Rangpur district, Bangladesh and then the leaves were air dried. The dried leaves were grinded with a mortar and preserved in air-tight bottles. About 50mg of the powered sample was taken in a 15 ml tube and mixed with 5ml methanol and shook for 4hrs at 25°C. About 1ml of the supernatant liquid was transferred into an Eppendorf tube and centrifuged. The clear solution was diluted 100 times for analysis. Results show that hybrid system gives much more abundant ions compared with ESI only. This suggests that non-polar components in extracts that are difficult to detect by ESI can be effectively ionized by barrier discharge ionization.