Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Meng Sun, Na Zheng, Yaling Shi and Wen Lu
Xi'an Jiaotong University, China
Posters & Accepted Abstracts: J Chromatogr Sep Tech
A quantitative method, namely high-performance liquid chromatography coupled with diode array and evaporative light scattering detectors (HPLC-DAD-ELSD), was developed for simultaneous determination of six active ingredients in astragali radix from 10 different areas and 5 compound preparations of different dosage form. The DAD wavelength at 254 nm was selected for UV detection of three isoflavonoids (1: calycosin-7-O-�?²-D-glycoside, 2: ononin, 3: calycosin), while the drift tube temperature at 90�?ºC and the nebulizing gas pressure at 1.5 bar were set for ELSD detection of three astragalosides (4: astragaloside IV, 5: astragaloside III and 6: astragaloside I). The conditions of this assay were optimized and the method was fully validated with respected to linear range, precision, repeatability and recovery. The developed method was successfully applied to determination six active ingredients in 15 samples and the results showed distinctive features of the contents of isoflavonoids and astragalosides. This rapid and reliable HPLC-DAD-ELSD method is suitable for quality evaluation of astragali radix and its compound preparations from different source and manufacturing procedure.
Email: sunmeng@mail.xjtu.edu.cn