Separation of hydrophobic amino acid enantiomers in CEC system by | 54184
Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

+44 1300 500008

Separation of hydrophobic amino acid enantiomers in CEC system by molecular imprinting technique

World Congress on Chromatography

September 21-23, 2016 Amsterdam, Netherlands

Koray Sarkaya, Suleyman As�?±r, Ali Derazhamshir, Fatma Y�?±lmaz, Handan Yavuz and Adil Denizli

Hacettepe University, Turkey
Abant Izzet Baysal University, Turkey
Middle East Technical University, Turkey
D�?¼zce University, Turkey

Scientific Tracks Abstracts: J Chromatogr Sep Tech

Abstract :

Capillary electrochromatography (CEC), first described by Pretorius, is a rapidly evolving hybrid technique between CE and LC. Analytes may be separated in CEC by the combined action of partitioning between stationary phase and mobile phase (chromatographic interactions) and by their difference in electrophoretic mobility (movement of solutes by electrical forces). Therefore, CEC usually possess higher efficiency and selectivity as compared with classical CE and LC. The use of CEC for chiral analysis has become popular in recent years due to high separation efficiency. Separation of enantiomers is very important because they may show different biological activities. Many single enantiomers of amino acids were also used for the synthesis of biologically important compounds. In this work, enantiomeric separation by CEC was extensively carried out using chiral monolithic capillary column. In this presentation, we will describe about the monolithic column, which is based on N-methacryloyl-(L)-phenylalanine methyl ester (MAPA) as hydrophobic monomer in CEC system.

Biography :

Koray �?�?arkaya is PhD student from Hacettepe University and has done his PhD studies from Biochromatography and Biodiagnostics Research Group of Hacettepe University. He has been a Research Assistant at the Düzce University since 2009.