Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

+44 1300 500008

Raman spectroscopy based method for the evaluation of compositional consistency of nanofibrous layers

World Congress on Chromatography

September 21-23, 2016 Amsterdam, Netherlands

Adela Kotzianova, Jiri Rebicek, Ondrej Zidek, Marek Pokorny, Jan Hrbac and Vladimir Velebny

Contipro Pharma a.s., Czech Republic
Masaryk University, Czech Republic
Contipro Biotech s.r.o., Czech Republic

Scientific Tracks Abstracts: J Chromatogr Sep Tech

Abstract :

Electrospinning is currently a very popular method used across a number of industries. Electrospinning enables the production of nanofibrous layers of various structures and compositions. The production of a multi-component nanofibrous layer may result in an uneven distribution of the individual components throughout the layer. Confocal Raman spectroscopy combined with statistical methods allows these layers to be analyzed by determining their chemical composition and thus provides feedback for the spinning process. This paper presents a method which combines Raman spectroscopy analysis and its subsequent evaluation with singular value decomposition (SVD). Automated measurement of Raman spectra makes it possible to gather extensive spectral data from a particular area selected on a sample; the spectra are measured from a specific volume and not from individual fibers. Samples require no preparation for the analysis and the non-destructive nature of Raman spectroscopy ensures their reusability. When spectra of the individual component materials are included for reference, the subsequent SVD analysis of the spectral data makes it possible to determine the chemical composition of the measured areas, thus providing the content percentages of the individual components, which can be displayed either in the form of a scattered plot or a Raman map.

Biography :

Adéla Kotzianová graduated from the Czech Technical University in Prague in 2012, where she earned her Master’s degree in Instruments and Methods for Biomedicine. Currently, she is finishing her Doctoral studies in Physical Chemistry at the Masaryk University in Brno. Since 2012, she has been working as a researcher in the Department of Nanotechnology Device Development of the Contipro Holding. She has been specializing in spectroscopic methods since completion of her studies. In recent years, her work was focused especially on the preparation and analysis of nanofibrous materials. She has published 7 papers in reputed journals and presented her work on international conferences.