Journal of Chromatography & Separation Techniques
Open Access

Quantitative proteomic analysis of wheat shoots in rain-fed and irrigated varieties under copper nanoparticles exposure

5^th International Conference on Current Trends in Mass Spectrometry and Chromatography

September 25-26, 2017 Atlanta, USA

Naveed Iqbal Raja, Farhat Yasmeen, Noshin Ilyas and Setsuko Komatsu

Pir Mehr Ali Shah Arid Agriculture University, Pakistan
National Agriculture and Food Research Organization, Japan

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Environmental factors influence the rate of rain fall which affected the growth of wheat. Copper (Cu) nanoparticles (NPs) have enhanced the germination and growth of wheat. To elucidate the effects of Cu NPs on shoot of rain-fed Pakistan-13 and irrigated NARC-11, quantitative proteomic technique was used. The weights and lengths of seedling shoot and root of wheat varieties were increased on 10 ppm Cu NPs exposure. The number of proteins related to protein metabolism was increased in rain-fed variety while protein metabolism and photosynthesis related proteins were increased in irrigated variety, treated with Cu NPs compared to untreated plants. Abundance of proteins related to glycolysis and tricarboxylic acid cycle was increased on Cu NPs exposure in Pakistan-13 and NARC-11. However, the abundance of proteins related to photosynthesis and tetrapyrrole synthesis was decreased on Cu NPs exposure in Pakistan-13 and NARC-11. Chlorophyll content such as chlorophyll a, chlorophyll b and total chlorophyll was decreased on Cu NPs exposure in Pakistan-13 and NARC-11. These results suggest that Cu NPs improve the growth of wheat seedling, which might be associated with the increase of protein abundance related to glycolysis and tricarboxylic acid cycle in wheat varieties.