Quantification of bilirubin on dry blood spot by tandem mass spec | 53339
Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

+44 1300 500008

Quantification of bilirubin on dry blood spot by tandem mass spectrometry

2nd International Conference on Current Trends in Mass Spectrometry

July 20-22, 2016 Chicago, USA

Zhenhua GONG

Children's Hospital affiliated with Shanghai Jiaotong University and Children's Hospital of Shanghai, China

Scientific Tracks Abstracts: J Chromatogr Sep Tech

Abstract :

Background: Hyperbilirubinemiais caused by many kind diseases and is harmful to neonate development, it is necessary to detecthyperbilirubinemia as early as possible, or probably detect biliary atresia (BA) earlier. A new method for screening and clinical determination of blood bilirubin on a dry blood spot (DBS) using tandem mass spectrometry (MS/MS) was developed. Methods: The serum bilirubin controls and blood were prepared as DBS, extracted into a methanol solution containing isotope-labeled external standards, were subjected to HPLC, detect by MS/MS. Multiple-reaction monitoring of m/z 585â�?�?299 for unconjugated bilirubin(UBIL), m/z761â�?�?585 for monoglucuronide bilirubin(BMG) and m/z 937â�?�?585 for diglucuronide bilirubin (BDG) were used to detect bilirubins. Results: The recoveries of UBIL by MS/MS were 90%-120.31% with an R2 value of 0.928 after linear regression (p<0.001). The coefficients of variation were less than 20%.The blood UBIL in neonates aged 3-7 days (68.14�?±21.56umol/L) was higher than in children aged older than 1 mouth(38.24�?±7.95umol/L). BMG and BDG were not higher in BA than control significantly. Conclusion: Quantification of UBIL on a dry blood spot by MS/MS is accurate, reliable and feasible for screening and clinical tests. There may be �?´-bilirubin caused direct bilirubin elevated in BA.

Biography :

Zhenhua GONG has completed his PhD at the age of 40 years from Fudan University,Shanghai China. He is pediatric surgeon, associated professor and director of a research team focusing on metabolic diseases and biliary atresia. He has published more than 10 papers in reputed journals and serving as an editorial board member of repute.