Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Lee Suan Chua
UniversitiTeknologi Malaysia, Malaysia
Posters & Accepted Abstracts: J Chromatogr Sep Tech
Edible bird��?s nest (EBN) is a delicacy and nutritious salivary secretion of swiftlets. It contains mostly protein and carbohydrate, and fat as a minor component. The proteome of EBN is still intensively investigated, mainly due to the recent scientific findings on its medical benefits and rejuvenation of skin complexion. In the present study, proteins from EBN samples were extracted by ultrasonic and detergent assisted techniques, as well as buffer solublization. Aqueous extraction assisted by ultrasonic energy produced the highest protein content, followed by detergent assisted method and buffer solubilization. A wide range of protein sizes ranging from 17-150 kDa, mostly water soluble proteins were detected from EBN samples based on gel electrophoresis. EBN from different locations were found to have slight variance in their electrophoretic protein profile. This could be due to the difference in food sources for swiftlets. The use of detergents such as non-ionic Triton X-100 and ionic SDS produced additional protein bands which could be membrane or transmembrane proteins. The trypsinized proteins were subjected to LC-MS/MS analysis and the mass spectra were matched to the protein database. Acidic mammalian chitinase precursor is the most abundant protein in EBN. The protein was reported to have anti-inflammatory activity against allergy and airway diseases. There are also collagen, lysyl oxidase homolog 3 and co-enzyme Q-binding proteins which are important for skin complexion. The results could explain the remarkable findings of previous investigators on the benefits of EBN consumption. These proteins are first time reported to be presence in EBN.
Email: chualeesuan@utm.my, lschua@ibd.utm.my