Platform chromatographic purification of AAV gene therapy vectors | 55973
Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

Platform chromatographic purification of AAV gene therapy vectors

4th World Congress on Chromatography

August 07-09, 2017 | Rome, Italy

Ales Strancar and R Sekirnik

BIA Separations, Slovenia

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Adeno-associated virus (AAV) vectors of various serotypes are considered to have high potential for gene therapy applications. Currently, manufacturing of AAV vectors faces the challenge of co-production of incompletely formed particles lacking a recombinant viral genome. Empty capsids increase the dose of total AAV administered for efficient transduction and are thought to cause unwanted immunological reactions against the virus. Removal of empty capsids during manufacturing, as well as analysis of empty/full AAV particle content is therefore a critical requirement for any AAV production process. Poster demonstrates how CIMmultus� QA monolithic columns can be used to remove empty AAV capsids from the product chromatographically in a single step.

Biography :

Aleš Štrancar is the CBDO of the BIA Separations and one of the main inventors of the CIM Convective Interaction Media®. He is author and co-author of more than 60 scientific papers dealing with separation and purification technologies, a co-author of five granted USA patents and their foreign equivalents in the field of biomolecule separations and purification and a co-author of several book chapters dealing with novel chromatography technologies for biomolecule separation. He co-developed several industrial scale purification processes. He was the President of Technology Council of Ministry of Economy of the Republic of Slovenia and Member of Science and Technology Council of the Republic of Slovenia.


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