Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Seyed Jamaleddin Shahtaheri
Tehran University of Medical Sciences, Iran
Scientific Tracks Abstracts: J Chromatogr Sep Tech
Introduction & Aim: Measurement of pesticides in biological matrices has become
a serious challenge for researchers due to their very low concentration in different
matrices. The aim of this study was to optimize a new sample preparation procedure
with high accuracy and validity and short retention time for determination of diazinon.
Materials & Methods: Dispersive liquid-liquid micro-extraction technique coupled
with high performance liquid chromatography equipped with ultra violet detector
was developed for trace extraction and determination of diazinon pesticide in human
urine samples. One variable at a time method was used to optimize parameters
affecting the diazinon extraction. Different parameters such as extraction solvent,
disperser solvent, and volume of the extraction solvent, volume of the disperser
solvent, centrifugation time and speed, salt addition, and sample pH were studied
and optimized.
Result: Findings showed that optimal levels of these variables for diazinon pesticide
were 150 μl of carbon tetrachloride as extraction solvent, 1.5 ml of methanol as
dispersive solvent, pH of 6, 5 minutes centrifuge time at speed of 4000 rpm , 0%
(w/v) salt addition. Correlation coefficient was 0.9965 indicating the linearity of
a wide range of concentrations of the toxin. LOD and LOQ was calculated less
than 0.7 and 5 μg L-1 respectively. The relative standard deviation for six replicate
experiments in intra-day and inter-day at three selected concentrations (50, 200
and 1000 μg L-1) was less than 4% that indicates the accuracy and precision of the
optimized method. Enrichment factor and extraction recovery for diazinon were 245
and 99% respectively.
Conclusion: According to the results, dispersive liquid-liquid micro-extraction
procedure was successfully developed for the extraction of diazinon from human
urine samples. Compared to other extraction techniques, the proposed procedure had
some advantages such as shorter extraction time, better reproducibility, and higher
enrichment factor.
Seyed Jamaleddin Shahtaheri has completed his PhD from Surrey University, Guildford, Surrey, England in 1996. He is an Academic Member of Department of Occupational Health Engineering, Tehran University of Medical Sciences, Iran; acting as the Dean Research Deputy at Institute for Environmental Research in the same University. He served as Persistent Organic Pollutant Review Committee (POPRC) Member under the Stockholm Convention, UNEP, UN during the period 2014-2018
E-mail: shahtaheri@tums.ac.ir