Novel methodology of detecting minimal residual disease in acute | 33268
Pediatrics & Therapeutics

Pediatrics & Therapeutics
Open Access

ISSN: 2161-0665

+44 1478 350008

Novel methodology of detecting minimal residual disease in acute leukemia by using FTIR spectroscopy

International Conference and Exhibition on Pediatric Oncology and Clinical Pediatrics

August 11-13, 2016 Toronto, Canada

Soad K Al Jaouni

King Abdulaziz University Hospital, KSA

Posters & Accepted Abstracts: Pediat Therapeut

Abstract :

Background: Previously examined the potential of conventional Fourier transform infrared spectroscopy (FTIR) in an attempt to detect specific biomarkers for discrimination between disease free and acute leukemia bone marrow samples. Aim: To assess the efficacy in detecting minimal residual disease in acute leukemia by FTIR. Patient and Methods: Case report, to predict early relapse, and distinguish disease free in remission (control) bone marrow samples, four bone marrow samples were obtained from an 11-year-old boy diagnosed as acute leukemia. Samples (1) and (2) for the patient in remission as diagnosed clinically and laboratory examination free, sample (3) diagnosed relapse in CNS, while sample (4) was taken post-chemotherapy induction. Result: The results revealed that characteristic band alterations arising from cellular protein, lipid, and DNA were identified in both control and diseased samples (relapse of ALL). There were specific changes that affected the secondary structure of proteins and appeared in the FTIR spectra confirmed with the second-derivative analysis. The overall protein structure in the control sample consisted primarily of a-helices, whereas in relapse ALL sample, it had a relatively high proportion of anti-parallel b-sheet protein constituents presumably due to leukemia. Different absorbance ratios for specific bands were calculated and plotted versus the patient��?s samples in different time intervals. There are significant fluctuations in the ratios under investigation, which can be attributed to the changes in the biomolecular structure between the control and relapsed samples. These ratios are all yield statistically significant differences in parameters that it can be used as an early predictor of minimal residual disease (MRD) in acute leukemia. Conclusions: Fourier transform infrared spectroscopy is an effective method in detecting minimal residual disease in acute leukemia. Further larger samples is indicated.

Biography :