Journal of Chromatography & Separation Techniques
Open Access

Newly developed UHPLC method for the determination of omeprazole in oral suspensions

2^nd International Conference and Expo on Separation Techniques

September 26-28, 2016 Valencia, Spain

Kucerova K, Reiska V, Matysova L and Kujovska Krcmova L

Charles University in Prague, Czech Republic
University Hospital in Hradec Kralove, Czech Republic

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Ultra-high performance liquid chromatography (UHPLC) presents relatively new technique providing exceptional separation efficiency. Connection of UHPLC with the core-shell particles as a stationary phase is very advantageus. The porous surface of coreâ�?�?shell particles allows faster mass transfer and reduce the swirl effect as well as the back pressure of column, UHPLC enables fast and senzitive analyses. The new separation method was developed on KinetexTM C18 column (50�?�?2.1 mm) with core-shell particle size of 1.7 �?¼m. The mobile phase was phosphate buffer (25 mM, pH 7.6) and acetonitrile in the ratio 74: 26 (v/v) with total time of analysis 2 minutes and internal calibration. Full validation was performed. The new method was used to monitor the stability of the active substance â�?�? omeprazole (proton pump inhibitor) in suspensions and will serve for monitoring of omeprazole stability in individually prepared oral suspensions for pediatric, elderly as well as critically ill patients having problems to wallow solid dosage form.
The study was supported by project SVV 260 292.

Biography :

Kucerova K has completed her Master’s degree from Charles University in Prague at Faculty of Pharmacy in Hradec Kralove. Presently, she studies Postgraduate Doctoral Course in Bioanalytical Chemistry at the same faculty. She is interested in liquid chromatography with UV detection and optimization of sample pretreatment.

Email: kucerok1@faf.cuni.cz