Journal of Chromatography & Separation Techniques
Open Access

New nanomaterials for enhancing detection of small molecules in cells by MALDI TOF mass spectrometry

4^th World Congress on MASS SPECTROMETRY

June 19-21, 2017 London, UK

E M Pena-Mendez, Kristina Hajtmanova, Lenka Kolarova, Jose Elias Conde, Ales Hampl, Petr Va�?�?hara and Josef Havel

Universidad de La Laguna, Spain
Masaryk University, Czech Republic
Masaryk University, Czech Republic
St. Anne��?s University Hospital, Czech Republic

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Matrix assisted laser desorption ionization mass spectrometry (MALDI MS) became a powerful platform for detection of small molecules in many analytical applications. However, in cell biology, precise detection of small molecules by MALDI MS is challenging due to high biological complexity of samples, unwanted interferences from salts and matrix, requirements of uniform sample deposition or simply minute amounts of the analytes. In the last years, the use of nanoparticles or nanocomposites as new matrices for improving the analysis of small molecules by MALDI TOF mass spectrometry demonstrated promising advantages. The metal organic frameworks (MOFs), graphene oxides (GO), and/or gold nanoparticles (AuNPS) represent new nanomaterials with unusual physico-chemical properties and great analytical potential as matrices for MALDI MS. In this work, we investigated properties of new generations of MOFs and their combinations with GO and AuNPs and demonstrated that they can be effective platforms for the adsorption of small biomolecules, facilitating the ionization in MALDI MS. Finally, application of MOFs, GO, AUNPs and/or their combinations significantly improved detection of small molecules by MALDI MS in complex experimental biochemical or biological systems, e.g. in cells.

Biography :

Email: empena@ull.edu.es