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Monitoring of chronic wound healing process via determination of | 54391
Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

Monitoring of chronic wound healing process via determination of arginine and its metabolites using HPLC-FD method


2nd International Conference and Expo on Separation Techniques

September 26-28, 2016 Valencia, Spain

Javorska L, Aufartova J, Piskackova H, Kujovska Krcmova L, Sobotka L, Solichova D and Solich P

Charles University in Prague, Czech Republic
University Hospital Hradec Kralove, Czech Republic

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Chronic wound is the skin defect persisting longer than 6 weeks or is frequent reoccurrence defect1. These wounds remain in a chronic inflammatory state failing a normal healing process patterns2. The incidence of the non-healing wounds impaired quality of life for the affected patients and leads to the time-consuming and high cost treatment3. The amino acid arginine plays important role in wound healing as the precursor of nitric oxide (NO), proline and polyamines affect all phases of the wound healing process4. Monitoring of arginine metabolism directly in the chronic wound could be another indicator of the chronic wound healing process and significantly improve the therapy. Our HPLC method with fluorescence detection enable determination of arginine, ornithine and citrulline in a fluid obtained from chronic wounds. Separation of amino acids was performed using C18 monolithic HPLC column. Sodium acetate buffer (solution A) and mixture of ACN and MeOH (solution B) were used as the mobile phase in gradient elution mode. Total time of analysis was 14 min including the columnwash step. The method was validated by testing its linearity, precision, accuracy, recovery, robustness and detection limit/ quantitation limit values. This method will be used for clinical research of the chronic wound healing process. The study was supported by the SVV 260 292, Project MH CZ-DRO (UHHK, 00179906), PRVOUK P37/12.
References 1. Wild, T, Rahbarnia, A, Kellner, M, Sobotka, L., Eberlein, T.: Nutrition, 26, 2010, 862 ��? 866.
2. Cabrera, CA, Ramos, AN, Loandos, MD, Valdez, JC, Cabral, MES.: Pharmaceutical development and technology, 21 (4), 2016, 399 ��? 404.
3. H�?¤mmerle G, Strohal R.: International Wound Journal, 13, 2016, 182 â�?�? 188.
4. Gould A, Naidoo C, Candy GP.: Wound healing Southerm Africa, 1, 2008, 48 ��? 50.

Biography :

Javorska L has completed her Master’s degree in 2013 from the Faculty of Pharmacy, Charles University in Prague. Since 2013, she is a PhD student at the Faculty of Pharmacy, Charles University in Prague. She is interested in LC chromatography with UV, FD, MS detection and sample preparation techniques. She has published 1 paper in reputed journal.

Email: javorskl@faf.cuni.cz

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