Molecular typing of Mycobacterium Leprae by variable number tande | 30694
Journal of Probiotics & Health

Journal of Probiotics & Health
Open Access

ISSN: 2329-8901

+44 1223 790975

Molecular typing of Mycobacterium Leprae by variable number tandem repeats in multi-case families of leprosy

4th International Conference and Exhibition on Probiotics, Functional and Baby Foods

November 03-05, 2015 Valencia, Spain

Idress Hamad Attitalla

Omar Al-Mukhtar University, Libya

Posters-Accepted Abstracts: J Prob Health

Abstract :

During the study period 2011-2013, 14 multi-case families have been identified from unique screening of 1084 houses from Andhra Pradesh and Odisha states. In all multi-case families, all first identified individuals were noted as MB. A total of 34 cases were successfully screened from multi-cases families, SSS samples were obtained and DNA was isolated and subjected to PCR-VNTR analysis. Families-1, 7, 11, 13 have copy number 4 and families-4, 14 have copy number 5 for locus (GGT) 5. Most of the families have copy number 2 for locus 21-3. The copy number 9 of (GTA) 9 has linked with households of family-1, 13, 14 and copy number 13 has seen in family 4. Copy number 11of (AT) 17 loci has coupled with households of family-1, 4 and copy number 12, 13 have associated with families 13 and 14. Copy number 8 of (AC) 9 locus has associate with households of family-1; 9 copy number in 4 and 14 families; 10 copy number in family13. For 6-7 locus, households of family-1, 3, 6 have 7 copy number; households of families -2, 5, 12, 13 and 14 have copy number 6; 5 copy number has seen in family-4. Households of family-1, 6, 8, 13 and 14 have 5 copy number; households of family-4 has copy number 4; 6 copy number has seen in family-5 and 12 for locus 27-5. 23-3 locus has copy number 2 in multi-case families. 12 copy number of (TTC) 21 loci have connected with households of families-1, 13 and 14; families-2 and 4 have copy number 10. Multi-case families those have same copy number of VNTR loci have been infected with leprosy patient in the households. The multi-case families whose have different copy number have infected with leprosy bacilli from the environment.

Biography :