Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
K S Nakhuru, J Gogoi, P Chattopadhyay and P S Raju
Defence Research Laboratory, India
Assam Down Town University, India
Posters & Accepted Abstracts: J Chromatogr Sep Tech
This study was carried out to isolate bioactive components from the roots of M. jalapa L. (Nyctaginaceae), which is widely used as folk remedy against numerous ailments such as carbuncles, urticaria, boils, ulcers, inflammation, etc. Aqueous methanolic extract was fractionated into four fractions according to polarity. Potential fractions identified with 2,2-diphenyl- 1-picrylhydrazyl (DPPH) radical scavenging capacity and activity against pathogenic bacterial and fungal strains. Thin-layer chromatography (TLC) was used for separation of bioactive components from potential fractions. Standards spotted alongside fractions of petroleum ether, chloroform and methanol (49:50:1) and chloroform, ethyl acetate and formic acid (5:4:1) was used as solvents for separation of terpenoids and flavonoids fractions, respectively. Chromatogram of terpenoids was developed with vanillin-sulfuric acid spray with heating, whereas, flavonoid spots were detected under ultraviolet rays at 365 nm. The relative front of each spot was calculated. One of the components of terpenoids corresponds to Ã?Â?-sitosterol. Each spot was further subjected to free radical scavenging and antimicrobial assays. TLC plate developed with 0.2% DPPH spray for detection of free radical scavenging spots. Susceptibility against pathogens such as Staphylococcus aureus, Streptococcus mutans, Escherichia coli, and Candida albicans was determined using agar well diffusion assay. Two spots, one from terpenoids fraction (5F1) and the other from flavonoids fraction (1F3), showed free radical scavenging capacity. 1F3 spot was active against both S. aureus and C. albicans, whereas, 5F1 spot was active against S. aureus alone. Our findings substantiated the use of MJ in folk remedy. Therefore, M. jalapa L. could be explored for potential source of free radical scavenger and/or broad spectrum antimicrobial agent against clinically important human pathogens.
Email: nakhuru12@gmail.com