HPLC-DAD-ESI-MS analysis of phenolic compounds from Eucalyptus gl | 52679
Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

HPLC-DAD-ESI-MS analysis of phenolic compounds from Eucalyptus globulus fruits

International Conference and Expo on Separation Techniques

August 10-12, 2015 San Francisco, USA

Lila Boulekbache-Makhlouf1, Emmanuelle Meudec2, Veronique Cheynier2, Jean-Paul Mazauric2, Sakina Slimani1 and Khodir Madani1

1University of Bejaia, Algeria 2INRA, France

Posters-Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

The present study consists on the characterization and evaluation of antioxidant activity of phenolic compounds of fruits of Eucalyptus globulus (E. globulus). A method based on high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) following fractionation by chromatography on a Sephadex LH-20 column has been developed. The presence of 18 gallotannins, 26 ellagitannins, and 2 flavonols was established. Tentative identification is provided for these compounds on the basis of UV-visible spectra and mass spectrometry data. Most compounds described in this study have not previously detected in fruit of E. globulus. Moreover, this is the first report of methyl digalloyl diglucose, 3, 3-0 -dimethylellagic acid 4- -�?²-glucopyranoside, ellagic acid hexose, methyl ellagic acid pentose, methyltetragalloylglucose, and valoneic acid isomers (sanguisorbic, flavogallic acid dilactone) in the genus. Quantitatively, ellagic acid and its derivatives, including ellagitannins, are largely predominant.

Biography :