Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Jose Licarion Pinto Segundo Neto
Laborat�?³rio de Automa�?§�?£o e Instrumenta�?§�?£o em Qu�?mica Anal�?tica e Quimiometria, Brazil
Posters & Accepted Abstracts: J Chromatogr Sep Tech
Biogenic amines are used for identifying spoilage in food. The most common are tryptamine, 2-phenylethylamine, putrescine, cadaverine and histamine. Due to lack of chromophores, chemical derivatization with dansyl was employed to analyze these amines using HPLC-DAD. However, the derivatization reaction occurs with any primary or secondary amine, leading to co-elution of analytes and interferents with identical spectral profiles, and thus causing rank deficiency. When the spectral profile is the same and peak misalignment is present on the chromatographic runs, it is not possible to handle the data only with MCR-ALS, by augmenting on the time, or the spectral mode. To overcome both problems, this paper proposes a new analytical methodology for fast quantitation of these BAs in fish with HPLC-DAD by using the icoshift algorithm for temporal misalignment correction before MCR-ALS spectral mode augmented treatment. Limits of detection, REP and average recoveries, ranging from 0.14 to 0.50 �?¼g mL-1, 3.5 o 8.8% and 88.08% to 99.68%, respectively. These results reaches quantification limits for the five BAs much lower than those established by FAO/WHO, and EFSA, all without any pre-concentration steps. The concentrations of BAs in fish samples ranged from 7.82 to 29.41 �?¼g g-1, 8.68 to 25.95 �?¼g g-1, 4.76 to 28.54 �?¼g g-1, 5.18 to 39.95 �?¼g g-1 and 1.45 to 52.62 �?¼g g-1 for TRY, PHE, PUT, CAD, and HIS, respectively. In addition, the proposed method spends less than 4 minutes in an isocratic run, consuming less solvent in accordance with the principles of green analytical chemistry.
Email: Licarion@gmail.com