Pediatrics & Therapeutics
Open Access
ISSN: 2161-0665
ISSN: 2161-0665
KunPeng Yao,
Chongqing Liangjiang New District Traditional Chinese Medicine Hospital, China.
Scientific Tracks Abstracts: Pediatr Ther
Objective: To investigate the expression of serum exosome let-7a and miR-155 and the changes of lung function indexes in children with acute attack of bronchial asthma, and to evaluate their prognostic value. Methods: A total of 60 children with acute onset of bronchial asthma admitted to the respiratory department or emergency department of our hospital from March 2021 to March 2023 were selected as the observation group, and 60 healthy patients who underwent physical examination during the same period were selected as the control group. The levels of let-7a and miR-155 in serum exosomes of the two groups were detected and compared by ELISA and RT- PCR, and the correlation between them and lung function indexes was analyzed by Spearman. The prognosis of the children in the observation group was followed up and divided into good prognosis group and poor prognosis group. The basic clinical data of the two groups were compared, and the risk factors affecting the prognosis of the children were analyzed by multivariate Logistic analysis. Receiver operating curve (ROC) was used to evaluate the predictive value of let-7a and miR-155 expression levels on the prognosis of children. Results: Compared with the control group, the level of let-7a in serum exosomes in the observation group was lower, while the expression of miR-155 was higher (P < 0.05). Spearman correlation analysis showed that FEV1/FVC and FEV1 were positively correlated with let-7a, while FEV1/FVC and FEV1 were negatively correlated with miR-155 (P < 0.05). After 1-month follow-up, 45 cases had good prognosis and 15 cases had poor prognosis. There were no significant differences in gender, age, BMI and history of allergic rhinitis between the good prognosis group and the poor prognosis group (P > 0.05). There were significant differences in the combined respiratory tract infection, FEV1 / FVC, FEV1, let-7a and miR-155 levels between the two groups (P <0. 05); Logistic regression analysis showed that let- 7a, miR-155, FEV1 / FVC and FEV1 were independent prognostic factors of children with acute attack of bronchial asthma (P < 0.05). ROC curve analysis showed that the area under the curve of let-7a, miR-155 and lung function in predicting poor prognosis was 0.657,0.682,0.701 and 0.726, respectively, and the area under the combined prediction curve was 0.803 (P< 0.05). Keywords: let-7a; miR-155; bronchial asthma; acute attack; serum exosome; pulmonary unction index; predictive value
Dr. KunPeng Yao is a highly experienced pediatrician with over 20 years of clinical practice. A graduate of Chongqing Medical University, he has trained at leading institutions, including the Children's Hospital of Chongqing Medical University. Specializing in pediatric respiratory, gastroenterology, cardiology, and neonatology, Dr. Yao has expertise in critical neonatal care. He has published two medical papers, holds two utility model patents (2022, 2023), and led a nationally significant project under the 14th Five-Year Plan, earning a national award certificate. With extensive clinical experience and a passion for advancing pediatric care, Dr. Yao continues to make a meaningful impact in his field.