Pediatrics & Therapeutics
Open Access

Establishment of the mouse model for neural tube defects induced by maternal nutrient inositol deficiency

3^rd Annual Summit on Clinical Pediatrics and Cardiology & Infancy, Child Nutrition and Development

October 16-18, 2017 New York, USA

Jianhua Wang, Jin Guo, Xiuwei Wang, Huixuan Yue, Shen Li, Fang Wang, Zhen Guan, Zhiqiang Zhu, Bo Niu and Ting Zhang

Capital Institute of Pediatrics, China

Scientific Tracks Abstracts: Pediatr Ther

Abstract :

Emerging findings from murine and epidemiological studies showed that periconceptional maternal nutrient inositol deficiency is one of the risk factor of neural tube defects (NTDs), but the mechanism is unclear. We established a maternal inositol deficiency NTDs mouse model for further using on the study of inositol deficiency related NTDs. C57BL/6 mice were injected with Li2CO3 (250 mg/kg-400 mg/kg body weight) which inhibit the key enzyme necessary for inositol synthesis and recycling (IMPase) on gestation day 7.5 (GD7.5). The myo-inositol levels were measured on 0 h, 4 h, 8 h and 16 h after injection respectively. With the increase in Li2CO3 doses, both the incidence of absorbed embryos and the rate of growth retardation increased. Rates of NTD embryos in each Li2CO3 group were different. The group treated with 350 mg/kg Li2CO3 had the highest rate of NTD (26.2%) and lowest lethality. The phenotype of NTDs included encephalocele (88.8%), anencephaly (7.4%) and spina bifida (3.7%). The inositol level of the pregnant mouse decreased significantly after the 350mg/kg Li2CO3 injection, and reached to the lowest inositol level 8 hours after Li2CO3 injection (P<0.01). Serial transverse sections of brain in GD13.5 NTD embryos displayed obvious constrictive and deformed third, fourth and lateral ventricle due to over proliferation. The treated non-NTDs group also showed obvious hyper proliferation in the brain tissue and displayed serious changes of the neuroepithelial cells. The maternal inositol deficiency induced NTDs mouse model was successfully established, which could provide the animal model for further investigate the mechanism of inositol deficiency on NTDs occurrence.

Biography :

Jianhua Wang is currently working at Capital Institute of Pediatrics, China. His research interests are pediatric nutrition, epidemiological and clinical studies, etc.