Accepted Abstracts: J Chromat Separation Techniq
C DT is a serum marker used to estimate chronic alcohol abuse. This assay is both difficult and critical for cirrhotic patients, especially those awaiting liver transplantations. In this presentation, we propose to display the performances of the CDT- Capillarys assay (Sebia) to discriminate abstainers from abusers among cirrhotic patients and to expose recommendations to improve its reliability in this patient group. Methods: 110 patients with known hepatic status of cirrhosis had their CDT measured by Capillarys2 and confronted to their daily alcohol intake. CDT assays by the Bio-Rad %CDT by HPLC test or the N-Latex CDT assay (Siemens) were also performed as alternative methods. Results: Many electrophoretic profiles displayed by the Capillarys2 are extensively processed by the Phoresis software in case of cirrhosis. This was not observed with control sera. In order to decide when the processed electrophoretic profiles could reliably be used, we defined a qualitative criterion. This criterion consisted to apply an indicator of the resolution between the disialoand trisialo- transferrin peaks. Thus detecting abusers with cirrhosis using the CDT-Capillarys assay is possible when the indicator is in the normal range. However, only 54% of the profiles from cirrhotic patients fulfilled this criteria and no alternative CDT assay demonstrated satisfying performance for excluded samples. Conclusions: Applying an internal criterion of quality can help deciding which electrophoretic profiles can reliably be interpreted. However, improving the global quality of the test and decreasing the number of uninterpretable results require an improvement of the CDT assay.
Gonzalo, P., Pecquet, M., Bon, C., Gonzalo, S., Radenne, S., Augustin-Normand, C., and Souquet, J.-C. (2012). Clinical performance of the carbohydrate-deficient transferrin (CDT) assay by the Sebia Capillarys2 system in case of cirrhosis. Interest of the Bio-Rad %CDT by HPLC test and Siemens N-Latex CDT kit as putative confirmatory methods.