Journal of Chromatography & Separation Techniques
Open Access

Development of FPSE-HPLC-UV Method for analysis of phenyltin compounds in environmental and canned food samples

5^th International Conference and Expo on Separation Techniques

October 23-25, 2017 | Paris, France

Heena and Ashok Kumar Malik

Punjabi University, India

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

This paper narrates a novel fabric phase sorptive extraction-high performance liquid chromatography-ultra visible detection (FPSE-HPLC-UV) method for the simultaneous extraction and analysis of four phenyltin derivatives that include triphenyltin hydroxide, triphenyltin acetate, triphenyltin chloride and tetraphenyltin in environmental (agricultural waste water and municipal waste water) and canned food sample. The selected analytes were well resolved by waters nova pack C18 column (3.9 x 150 mm, 4�?µm) in isocratic elution mode within 15 minutes. The new microextraction media has been analytically evaluated using phenyltin derivatives as model compounds. The factors affecting the extraction efficiency of FPSE have been probed and the optimized extraction conditions have been determined. Under these optimum conditions, the limits of detection (LODs) for sol-gel C18 coated FPSE media in combination with HPLC-UV for the analysis of the phenyltin derivatives were in the range of 10-100 ng/mL with precision (relative standard deviation) at 10 ng/mL concentration with good absolute recoveries and less relative standard deviation. To the best of our knowledge, this is the pioneer FPSE extraction procedure, applied on environmental and canned food sample for the simultaneous determination of phenyltin derivatives and could be mimic as a rapid and robust green analytical tool.