Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Angela Santos-Fandila
Posters-Accepted Abstracts: J Chromatogr Sep Tech
The aim of this talk is to promote the use of Ultra-High Performance Liquid Chromatography coupled to Mass Spectrometry in tandem (UHPLC-MS/MS) in different areas and fields of science to cope with analytical problems of diverse nature. The high value and interest of this technique resides in the potential and versatility that it has, covering a wide range of possibilities from analytical support for research groups to production and quality control in the oil and food industries, or to hospitals and the healthcare sector, forensic and toxic laboratories, etc. The selectivity, specificity and high sensitivity have made that the fields of application of this technique are experiencing a rapid growth in recent years. In particular, in this talk, several applications in health sciences are presented to determine specifically important biomarkers modified by nutritional intervention. The difficulty with this type of application is mainly the validation of analytical methodology to obtain results of high confidence. The analytical methodology applied to biological systems has difficulties to provide a typical validation. The analytical methods presented in this summary have been validated under recommendations of the international guidelines for bioanalytical methods such as �??Guidance for Industry, Bioanalytical Method Validation, US (2001) of FDA. This guide admits some difficulties such as lack of certified standards, reference materials or the inability to perform interlaboratory comparative determinations, being less demanding and strict than other international regulations aimed at routine laboratories in industrial systems such as the ISO 17025. Several examples of analytical methods to determine biomolecules of important roles in human biochemistry will be presented in this speech. These analyzed biomolecules by UHPLC-MS/MS are interesting in the nutritional industry to be candidates as innovative products focused on cognitive functions, improvements and development in muscle-bone system, neuroimmunology reinforcement, etc. In sum, UHPLC-MS/MS is a powerful tool for a research group focused on discovering new ingredients or technologies that could be applied to new nutritional products or improve those existing already in the market, to avoid or mitigate disease and ailments that affect today�??s society.