Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Ravikanth Danda1, Kalaivani Ganapathy1, Keshava Prasad2, Harsha Gowda2, Akhilesh Pandey3, Subramanian Krishnakumar1 and Sailaja V Elchuri1
Posters-Accepted Abstracts: J Chromatogr Sep Tech
Retinoblastoma (RB) is an intraocular cancer found in children and occurs due to the inactivation of both alleles of retinoblastoma (RB1) gene located at the 13q14 region of chromosome 13. Abnormality/loss of RB1 gene initiates retinoma and genomic instability which primarily leads to retinoblastoma and is also involved in the progression of other cancers. We performed a high throughput comparative proteomic study to identify differentially expressed proteins in retinoblastoma using iTRAQ labeling and Orbitrap mass spectroscopy. In the present study we report first comprehensive proteomic signature of Retinoblastoma. Proteomic atlas was obtained by proteomic analysis of Total proteome, Membrane proteome and Phosphoproteome using Retina and retinoblastoma primary tumor. We identified 897 differentially expressed proteins in the total proteome out of which 398 are were upregulated and 499 were down regulated. Selective novel proteins from the mass spectrometry were evaluated on individual tumors by immunohistochemistry. CHGA, AHSG, ApoA2 IGF2BP1 and MDK were up regulated in Retinoblastoma. Knock down studies of novel identified protein revealed IGF2BP1 as a potential target molecule in retinoblastoma. We identified 931 differential proteins in membrane proteome out of which 419 were upregulated and 512 were down regulated. Two novel membrane proteins in the context of retinoblastoma LMNB1 and TFRC are validated using immunohistochemistry in primary tumors. Pathway analysis reveals dysregulation of lipid metabolism and photo transduction pathways. In conclusion our study provides comprehensive proteomic atlas of Retinoblastoma.