Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Mohamed Ibrahim Badaoui, Mohamed Benkhaled and Hamada Haba
Batna-1 University, Algeria
Posters & Accepted Abstracts: J Chromatogr Sep Tech
The research of active principles extracted from the aromatic and medicinal plants is of a key importance, because it allows the development of drugs to maintain the health of the human being. It is for this reason that we are interested in this type of investigation in our research laboratory. Besides, our laboratory has recently started a research program intended for the systematic study of medicinal plants belonging to the genus Atractylis (Asteraceae family) in view of their valorization. The present study focuses mainly on the species Atractylis cancellata. The Asteraceae family is characterized by its wealth in secondary metabolites of biological interest such as the triterpenes, the phenolic compounds of type flavonoids, and sesquiterpenes. The plants of the genus Atractylis are deemed in traditional medicine for treating many diseases. The main purpose of our work is to isolate and identify secondary metabolites that may have a biological activity from the different organic extracts of the species A. cancellata. The hydroalcoholic extraction: alcohol/water, separation and purification chromatographic: VLC, CC and TLC of extracts petroleum ether and ethyl acetate acquired from the whole plant A. cancellata, allowed us to isolate and identify secondary metabolites of different types flavonoids, sterols and triterpenes. The determination of their molecular structures is performed by the spectroscopic analysis methods such as the NMR 1D (1H, 13C J-modulated and DEPT) and the NMR 2D (COSY, HSQC, HMBC, and NOESY) and the mass spectrometry ESI-MS. It is mainly of lupeol, oleanolic acid, �?²-sitosterol and apigenin.